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KMID : 0379119970250010001
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Korean Journal of Mycology
1997 Volume.25 No. 1 p.1 ~ p.5
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Isolation and Sequencing of the cDNA Encoding β-tubulin from Pleurotus sajor - caju
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박수철/Park, Soo Chul
류진창/권석태/신평균/김범기/유영복/정미정/Ryu, Jin Chang/Kwon, Suk Tae/Shin, Pyung Gyun/Kim, Beom Gi/Yoo, Young Bok/Jeong, Mi Jeong
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Abstract
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The cDNA encoding β-tubulin of Pleurotus sajor-caju was isolated using an internal gene segment probe amplified by polymerase chain reaction (PCR) of genomic DNA and by cDNA library screening. The cDNA was consisted of 1560 nucleotides(nt), including a 5´-untranslation region (UTR) of 27nt, an open reading frame (ORS of 1341nt, and a 3´-UTR of 191nt. The ORF encoded a protein of 446 amino acids(aa), which shows over 80% homology with β-tubulins of other filamentous fungi. Southern hybridization analysis showed that there were two isotypes of β-tubulin genes in P. sajor-caju. Through sequence analysis we found that β-tubulin had a unusual Cys^(165) residue, which might be a significant factor for the insensitivity of fungi to fungicide benomyl.
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